Journal: The Journal of Biological Chemistry
Article Title: The Nicotinic Acetylcholine Receptor and the Na,K-ATPase ?2 Isoform Interact to Regulate Membrane Electrogenesis in Skeletal Muscle *
doi: 10.1074/jbc.M110.150961
Figure Lengend Snippet: Changes in the resting membrane potential of rat diaphragm muscle induced by QX-222. Changes in membrane potential produced by 50 μm QX-222 alone followed by the addition of 100 nm ACh (open circles), and 50 μm QX-222 in the presence of 50 nm ouabain (closed circles; ouabain incubation was started 30 min prior to the test solutions). Horizontal bars indicate the periods when QX-222, ACh, or ouabain was present. Membrane potentials were recorded from extrajunctional membrane regions.
Article Snippet: ACh, ouabain, proadifen, QX-222, tetracaine, and nicotine ((−)nicotine hydrogen tartrate), and diisopropyl fluorophosphates were obtained from Sigma. α-Bungarotoxin was from Molecular Probes (Eugene, OR) and [ 3 H]ouabain was obtained from Amersham Biosciences.
Techniques: Produced, Incubation